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KMID : 0380019950100020131
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Korean Journal of Biotechnology and Bioengineering
1995 Volume.10 No. 2 p.131 ~ p.136
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Studies of Producing Anti-microbial Factor from Human Promyelocytic Cells
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Murakami H.
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Abstract
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0.374(1/day) of specific growth rate and 0.435(mg/108 viable cells) of Anti-Microbial Factor (AMF) productivity were observed for the batch cultivation of human promyelocytic cells in 10% serum containing medium. The crude protein was purified 10 folds by a serial purification steps of ion exchange chromatography, Bio-Rex 70 and gel filtration chromatography, Sephadex G-70 and 100. The ranges of MIC(Minimal Inhibitory Concentration) of commercially available antibiotics, penicillin G, streptomycin and ampicillin was estimated as 40 to 70 (ug/ml) on Gram (-) E. coli and Gram (+) Streptococcus aureus. The values of the MBC (Minimal Bactericidal Concentration) of purified AMF was 0.5(ug/ml) and 0.4 (ug/ml), respectively. The molecular weight of the AMF was estimated as 15, 000 dalton by SDS-PAGE.
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